Cloned (Comment) | Organism |
---|---|
expressed in Sf9 insect cells, in CHO cells, in human renal proximal tubular epithelial (HK-2) cells, and in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350, 10% (v/v) ethylene glycol, 0.2 M NaBr, 0.1 M Bis-Tris propane (pH 6.6) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
2-nonenal | pH and temperature not specified in the publication | Homo sapiens | |
0.0175 | - |
octanal | pH and temperature not specified in the publication | Homo sapiens | |
0.0285 | - |
n-nonanal | pH and temperature not specified in the publication | Homo sapiens | |
0.0391 | - |
hexanal | pH and temperature not specified in the publication | Homo sapiens | |
0.0411 | - |
Betaine aldehyde | pH and temperature not specified in the publication | Homo sapiens | |
0.169 | - |
alpha-aminoadipate semialdehyde | pH and temperature not specified in the publication | Homo sapiens | |
0.5302 | - |
benzaldehyde | pH and temperature not specified in the publication | Homo sapiens | |
0.6474 | - |
propanal | pH and temperature not specified in the publication | Homo sapiens | |
7.374 | - |
glyceraldehyde | pH and temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
mitochondrion | - |
Mus musculus | 5739 | - |
additional information | not detected in microsomes | Mus musculus | - |
- |
nucleus | - |
Mus musculus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
MALDI-TOF mass spectrometry | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography and Superdex S200 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bladder | - |
Mus musculus | - |
brain | highest ALDH7A1 expression | Mus musculus | - |
colon | - |
Mus musculus | - |
duodenum | - |
Mus musculus | - |
eye | - |
Mus musculus | - |
heart | - |
Mus musculus | - |
ileum | - |
Mus musculus | - |
jejunum | - |
Mus musculus | - |
kidney | highest ALDH7A1 expression, ALDH7A1 in the kidney localizes to endothelial and podocyte cell nuclei | Mus musculus | - |
liver | highest ALDH7A1 expression | Mus musculus | - |
lung | - |
Mus musculus | - |
pancreas | highest ALDH7A1 expression | Mus musculus | - |
stomach | - |
Mus musculus | - |
testis | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nonenal + NAD+ + H2O | - |
Homo sapiens | (E)-non-2-enoate + NADH + H+ | - |
? | |
alpha-aminoadipate semialdehyde + NAD+ + H2O | - |
Homo sapiens | alpha-aminoadipic acid + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | low affinity for benzaldehyde | Homo sapiens | benzoate + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | - |
Homo sapiens | betaine + NADH + H+ | - |
? | |
glyceraldehyde + NAD+ + H2O | - |
Homo sapiens | glycerate + NADH + H+ | - |
? | |
hexanal + NAD+ + H2O | high specificity | Homo sapiens | hexanoate + NADH + H+ | - |
? | |
n-nonanal + NAD+ + H2O | high specificity | Homo sapiens | n-nonanoate + NADH + H+ | - |
? | |
octanal + NAD+ + H2O | high specificity | Homo sapiens | octanoate + NADH + H+ | - |
? | |
propanal + NAD+ + H2O | - |
Homo sapiens | propionate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase 7A1 | - |
Mus musculus |
aldehyde dehydrogenase 7A1 | - |
Homo sapiens |
Aldh7a1 | - |
Mus musculus |
Aldh7a1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus | |
NAD+ | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | ALDH7A1 knockdown sensitizes human renal proximal tubular epithelial cells and CHO cells to hyperosmotic stress | Homo sapiens |
physiological function | ALDH7A1 is involved in cellular defense against hyperosmotic stress | Mus musculus |
physiological function | ALDH7A1 is involved in cellular defense against hyperosmotic stress | Homo sapiens |